PRGF Induces MSC Osteoblastic Differentiation via BMP-2 and wnt Non-canonical Pathway

Introduction Platelet-rich-plasma (PRP) is autologous plasma that has a platelet concentration above baseline. Upon activation, PRP releases a high concentration of various growth factors. Platelet -released growth factors (PRGF) have been used to support the repair of bone defects, especially in maxillofacial and dental surgery, yet their exact effect on bone healing is not clear. We previously showed a significant osteoinductive effect of PRGF on Mesenchymal Stem Cells (MSC), as shown by an increased expression of typical osteoblastic marker genes and matrix mineralization. In particular, we showed that PRGF up-regulates BMP2 at both gene and protein level. Furthermore, our observations suggested the possible involvement of the Wnt-signalling cascade. In the present work, we further investigate the intracellular pathways leading to MSCs osteogenic differentiation under PRGF stimulation, compared to the classical osteogenic media (Dexamethasone and BMP2 containing media).